Home > Immunology Assays > Human Microglia
Induced microglia phenotypeHuman monocytes cultured in a CNS cytokine cocktail induces differentiation to a microglial phenotype. Human monocytes can be purified from the peripheral blood of healthy volunteers or from patients. A range of assays can then be performed including phagocytosis, cytokine release, NFKB translocation, and others customised to the target of interest. All assays can be performed in 96- or 384- well formats.
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Further Immunology Assays
B Cells Haemolysis Testing Macrophages/Monocytes Neutrophils/Granulocytes Phagocytosis Assays Regulatory T cells Spheroid Killing Assays Suppression Assays T Cell Activation Assays T Cell Exhaustion Assays Tumour infiltrating lymphocyte (TIL) and dissociated tumour cell assays Tumour Cell Killing Assays |
Phagocytosis of a Myelin Basic Protein Membrane Preparation
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Microglia (differentiated from peripheral blood monocytes) phagocytosing pH-rodo-labelled myelin basic protein (red when intracellular) over 14 hrs.
Other phagocytosis assays
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Phagocytosis of a range of other cargos such as pH-rodo labelled E.coli or labelled aggregated proteins can be performed. For example, the images below show cell association and uptake of Ab oligomers and fibrils, with quantification of lysosomal (LAMP-1) localisation.
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Cytokine releaseLevels of a wide range of cytokines and soluble factors (including IL-1b, IL-6, TNFa etc.) can be quantified by either ELISA or Luminex.
Cytokine release can be triggered by a range of PAMPs (e.g. LPS) or DAMPs (e.g. ATP) in addition to protein aggregates (e.g. Ab fibrils), enabling the assessment of test molecule impact on neuroinflammatory readouts. |
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NFκB translocation
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NFkB translocation provides an early indication of microglial activation and is suitable for profiling the action of test molecules against a range of stimuli.
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