Home > Immunology Assays > Regulatory T cells
Testing modulators or inducers of regulatory T cellsRegulatory T cells (‘Tregs’) play an important role in maintaining immune homeostasis and are capable of inhibiting the function of a large range of effector immune cells. Within the tumour microenvironment, regulatory T cells may expand and/or increase their suppressive function and so prevent anti-tumour responses (also see Suppression Assays pages for other cell types). In autoimmune diseases, expansion and/or enhancement of regulatory T cell populations may restore immune homeostasis.
Celentyx have developed several in vitro assays for interrogating regulatory T cell function. Human regulatory T cells can be purified from the blood of healthy volunteers or patients by magnetic-bead-based separation or FACS (important for the study of regulatory T cell sub-populations), followed by setup of cultures with effector cells in plate-based formats for therapeutic testing. Typical readouts include flow cytometry, ELISA, Luminex and high-content imaging. |
Further Immunology Assays
Antigen-specific Assays B Cells Fibroblasts Haemolysis Testing Human Microglia Macrophages/Monocytes Neutrophils/Granulocytes NK Cells Phagocytosis Assays Spheroid Killing Assay Suppression Assays T Cell Activation Assays T Cell Exhaustion Assays TIL and dissociated tumour cell assays Tumour Cell Killing Assays |
1: Human regulatory T cell induction, expansion and phenotyping
Regulatory T cell sub-populations exhibit differential expansion potential, suppressive function and survival. Purification by magnetic beads or fluorescence-activated cell sorting (FACS) enables properties of regulatory T cell sub-populations to be studied in detail.
Regulatory T cell sub-populations exhibit differential expansion potential, suppressive function and survival. Purification by magnetic beads or fluorescence-activated cell sorting (FACS) enables properties of regulatory T cell sub-populations to be studied in detail.
2. Regulatory T cell function
The suppressive function of regulatory T cells can be quantified in the absence or presence of test agents by titration of regulatory T cells in the presence of an activation stimulus (such as dendritic cells or anti-CD3/CD28 beads) and an effector/responder cell type. Suppression of activation marker expression, proliferation or cytokine production can then be quantified within the effector/responder population. Also see our dedicated Suppression assay page.
The suppressive function of regulatory T cells can be quantified in the absence or presence of test agents by titration of regulatory T cells in the presence of an activation stimulus (such as dendritic cells or anti-CD3/CD28 beads) and an effector/responder cell type. Suppression of activation marker expression, proliferation or cytokine production can then be quantified within the effector/responder population. Also see our dedicated Suppression assay page.
