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High-throughput confocal fluorescence microscopy of B cells, cancer cells, fibroblasts, epithelial cells, macrophages, microglia, NK cells, T cells and others

Celentyx Imaging Platform

Range of cutting-edge microscopy platforms available
Imaging platform can be applied to patient cells and tissues from a wide range of indications.
Assays can be performed in 96- or 384-well format.
Kinetic, live cell readouts possible (including high-speed imaging).
Multi-colour (4-colour imaging routinely performed; increased colours possible).
Labelling using antibodies, dyes, probes and reporters.
Automated image analysis performed in 2D or 3D.

Microglia (differentiated from peripheral blood monocytes) phagocytosing pH-rodo-labelled myelin basic protein (red when intracellular)

EXAMPLES OF APPLICATIONS INCLUDE:

Macrophage Phagocytosis Assays:

Human monocyte-derived macrophages phagocytosing pH-rodo labelled E.coli
Screening for phagocytosis modulators
High-content imaging screen for modulators of tumour cell phagocytosis with subsequent validation in orthogonal assays
Phagocytosis of patient CLL cells by human monocyte-derived macrophages

Induced Microglia Phagocytosis Assays

Human peripheral blood monocytes are cultured with a CNS cytokine cocktail to induce differentiation to a microglial phenotype
Phagocytosis of a myelin basic protein membrane preparation
Modulation of myelin basic protein uptake by small molecules

Fibrosis Assays

Immune Cell Killing

Receptor and Therapeutic Molecule Trafficking

A range of fluorescence microscopy techniques can be applied to track and quantify the movement of therapeutics or receptors within cells

Target Validation Support

Measurement of target expression in patient tissue by immunofluorescence or RNA detection

In addition to our standard assays, Celentyx is often called upon to develop bespoke assays.