Selected Publications from Celentyx Scientists
T cells/Immune checkpoints
B Cells
Antibody Engineering
Macrophages/Dendritic Cells
NK Cells
Fibroblasts/Fibrosis
Mast Cells/Eosinophils
Neuroscience/Pharmacology
Receptor Trafficking
B Cells
Antibody Engineering
Macrophages/Dendritic Cells
NK Cells
Fibroblasts/Fibrosis
Mast Cells/Eosinophils
Neuroscience/Pharmacology
Receptor Trafficking
T cells/Immune checkpoints
T regulatory cells as a potential therapeutic target in psychosis? Current challenges and future perspectives.
Corsi-Zuelli F, Deakin B, de Lima MHF, Qureshi O, Barnes NM, Upthegrove R, Louzada-Junior P, Del-Ben CM. Brain Behav Immun Health. 2021 Aug 19;17:100330. doi: 10.1016/j.bbih.2021.100330. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260954
Regulation of CTLA-4 recycling by LRBA and Rab11
Janman D, Hinze C, Kennedy A, Halliday N, Waters E, Williams C, Rowshanravan B, Hou TZ, Minogue S, Qureshi OS, Sansom DM. Immunology. 2021 Sep;164(1):106-119. doi: 10.1111/imm.13343. Epub 2021 Jun 6. PMID: 33960403; PMCID: PMC8358724.
Ex vivo modelling of PD‑1/PD‑L1 immune checkpoint blockade under acute, chronic, and exhaustion‑like conditions of T‑cell stimulations.
Roberts A, Bentley L, Tang T, Stewart F, Pallini C, Juvvanapudi J, Wallace GR, Cooper AJ, Scott A, Thickett D, Lugg ST, Bancroft H, Hemming B, Ferris C, Langman G, Robinson A, Chapman J, Naidu B, Pinkney T, Taylor GS, Brock K, Stamataki Z, Brady CA, Curnow SJ, Gordon J, Qureshi OS & Barnes NM. Sci Rep 11, 4030 (2021). https://doi.org/10.1038/s41598-021-83612-3
Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process.
Davies SP, Reynolds GM, Wilkinson AL, Li X, Rose R, Leekha M, Liu YS, Gandhi R, Buckroyd E, Grove J, Barnes NM, May RC, Hubscher SG, Adams DH, Huang Y, Qureshi O, Stamataki Z. Cell Rep. 2019 Nov 5;29(6):1610-1620.e4. doi: 10.1016/j.celrep.2019.09.068. PMID: 31693899; PMCID: PMC7057271.
Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells. Hou TZ, Qureshi OS, Sansom DM. Methods Mol Biol. 2019;1899:87-101. doi: 10.1007/978-1-4939-8938-6_7. PMID: 30649767.
Cytokine production and antigen recognition by human mucosal homing conjunctival effector memory CD8+ T cells.
Williams GP, Pachnio A, Long HM, Rauz S, Curnow SJ. Invest Ophthalmol Vis Sci. 2014 Nov 13;55(12):8523-30. doi: 10.1167/iovs.14-15133. http://iovs.arvojournals.org/article.aspx?articleid=2212722
Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4.
Qureshi OS, Zheng Y, Nakamura K, Attridge K, Manzotti C, Schmidt EM, Baker J, Jeffery LE, Kaur S, Briggs Z, Hou TZ, Futter CE, Anderson G, Walker LS, Sansom DM.Science. 2011 Apr 29;332(6029):600-3. doi: 10.1126/science.1202947. Epub 2011 Apr 7. http://science.sciencemag.org/content/332/6029/600.long
A transendocytosis model of CTLA-4 function predicts its suppressive behavior on regulatory T cells.
Hou TZ, Qureshi OS, Wang CJ, Baker J, Young SP, Walker LS, Sansom DM. J Immunol. 2015 Mar 1;194(5):2148-59. doi: 10.4049/jimmunol.1401876. Epub 2015 Jan 28. http://www.jimmunol.org/content/194/5/2148.long
CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells.
Restorick SM, Durant L, Kalra S, Hassan-Smith G, Rathbone E, Douglas MR, Curnow SJ. Brain Behav Immun. 2017 Aug;64:71-79. doi: 10.1016/j.bbi.2017.03.008. Epub 2017 Mar 20. https://doi.org/10.1016/j.bbi.2017.03.008
Inhibition of T cell apoptosis in the aqueous humor of patients with uveitis by IL-6/soluble IL-6 receptor trans-signaling. Curnow SJ, Scheel-Toellner D, Jenkinson W, Raza K, Durrani OM, Faint JM, Rauz S, Wloka K, Pilling D, Rose-John S, Buckley CD, Murray PI, Salmon M. (2004) J. Immunol 173:5290-5297. www.jimmunol.org/content/jimmunol/173/8/5290.full.pdf
B Cells
Selective serotonin reuptake inhibitors directly signal for apoptosis in biopsy-like Burkitt lymphoma cells.
Serafeim A, Holder MJ, Grafton G, Chamba A, Drayson MT, Luong QT, Bunce CM, Gregory CD, Barnes NM, Gordon J.(2003) Blood 101:3212-3219. www.bloodjournal.org/content/101/8/3212/tab-figures-only?sso-checked=true
Tone sets the signaling pathways and transcriptome generated in model CD40-pluricompetent L3055 Burkitt's lymphoma cells.
Stewart R, Wei W, Challa A, Armitage RJ, Arrand JR, Rowe M, Young LS, Eliopoulos A and Gordon J.(2007) Journal of Immunology 179:2705-2712 pdfs.semanticscholar.org/64c1/4b9799799e57b9fd33f7ee640f3b9f6b7843.pdf
High sensitivity and specificity of elevated cerebrospinal fluid kappa free light chains in suspected multiple sclerosis.
Hassan-Smith G, Durant L, Tsentemeidou A, Assi LK, Faint JM, Kalra S, Douglas MR, Curnow SJ. J Neuroimmunol. 2014 Nov 15;276(1-2):175-9. doi: 10.1016/j.jneuroim.2014.08.003. Epub 2014 Aug 8. https://doi.org/10.1016/j.jneuroim.2014.08.003
Novel vitamin D analogues; cytotoxic and anti-proliferative activity against a diffuse large B-cell lymphoma cell line and B-cells from healthy donors.
Kozielewicz P, Grafton G, Kutner A, Curnow SJ, Gordon J, Barnes NM. (2016) J Steroid Biochem Mol Biol. 164:98–105 http://europepmc.org/abstract/med/26485664 europepmc.org/abstract/med/26485664
Antibody Engineering
Fc-engineered antibodies with immune effector functions completely abolished.
Wilkinson I, Anderson S, Fry J, Julien LA, Neville D, Qureshi O, Watts G, Hale G. PLoS One. 2021 Dec 21;16(12):e0260954. doi: 10.1371/journal.pone.0260954. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260954
Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.
Qureshi OS, Rowley TF, Junker F, Peters SJ, Crilly S, Compson J, Eddleston A, Björkelund H, Greenslade K, Parkinson M, Davies NL, Griffin R, Pither TL, Cain K, Christodoulou L, Staelens L, Ward E, Tibbitts J, Kiessling A, Smith B, Brennan FR, Malmqvist M, Fallah-Arani F, Humphreys DP. Sci Rep. 2017 Dec 6;7(1):17049. doi: 10.1038/s41598-017-17255-8. https://www.nature.com/articles/s41598-017-17255-8
Macrophages/Dendritic Cells
Aqueous humor suppression of dendritic cell function helps maintain immune regulation in the eye during human uveitis.
Denniston AK, Tomlins P, Williams GP, Kottoor S, Khan I, Oswal K, Salmon M, Wallace GR, Rauz S, Murray PI, Curnow SJ. Invest Ophthalmol Vis Sci. 2012 Feb 21;53(2):888-96. doi: 10.1167/iovs.11-8802. Print 2012 Feb. http://iovs.arvojournals.org/article.aspx?articleid=2188371
Regulation of P2X4 receptors by lysosomal targeting, glycan protection and exocytosis.
Qureshi OS, Paramasivam A, Yu JC, Murrell-Lagnado RD. J Cell Sci. 2007 Nov 1;120(Pt 21):3838-49. Epub 2007 Oct 16. http://jcs.biologists.org/content/120/21/3838
ATP-induced autophagy is associated with rapid killing of intracellular mycobacteria within human monocytes/macrophages.
Biswas D, Qureshi OS, Lee WY, Croudace JE, Mura M, Lammas DA. BMC Immunol. 2008 Jul 15;9:35. doi: 10.1186/1471-2172-9-35. https://bmcimmunol.biomedcentral.com/articles/10.1186/1471-2172-9-35
NK Cells
Inosine Acedoben Dimepranol promotes an early and sustained increase in the natural killer cell component of circulating lymphocytes: A clinical trial supporting anti-viral indications.
Ahmed SR, Newman AS, O'Daly J, Duffy S, Grafton G, Brady CA, Curnow SJ, Barnes NM, Gordon J. (2017) Int. Immunopharmacol. 42:108–114. https://doi.org/10.1016/j.intimp.2016.11.023
Fibroblasts/Fibrosis
ILB® resolves inflammatory scarring and promotes functional tissue repair.
Hill LJ, Botfield HF, Begum G, Qureshi O, Vigneswara V, Masood I, Barnes NM, Bruce L, Logan A. NPJ Regen Med. 2021 Jan 7;6(1):3. doi: 10.1038/s41536-020-00110-2. https://www.nature.com/articles/s41536-020-00110-2
An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix.
Qureshi OS, Bon H, Twomey B, Holdsworth G, Ford K, Bergin M, Huang L, Muzylak M, Healy LJ, Hurdowar V, Johnson TS. Biol Open. 2017 Oct 15;6(10):1423-1433. doi: 10.1242/bio.025866. http://bio.biologists.org/content/6/10/1423 bio.biologists.org/content/6/10/1423
Quantitative and organisational changes in mature extracellular matrix revealed through high-content imaging of total protein fluorescently stained in situ.
Holdsworth G, Bon H, Bergin M, Qureshi O, Paveley R, Atkinson J, Huang L, Tewari R, Twomey B, Johnson T. Sci Rep. 2017 Aug 30;7(1):9963. doi: 10.1038/s41598-017-10298-x. https://www.nature.com/articles/s41598-017-10298-x. www.nature.com/articles/s41598-017-10298-
Mast Cells/Eosinophils
Human lung mast cells modulate the functions of airway smooth muscle cells in asthma.
Alkhouri H, Hollins F, Moir LM, Brightling CE, Armour CL, Hughes JM. Allergy. 2011 Sep;66(9):1231-41. doi: 10.1111/j.1398-9995.2011.02616.x. Epub 2011 May 10. https://doi.org/10.1111/j.1398-9995.2011.02616.x
Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P. J Immunol. 2008 Aug 15;181(4):2772-80 www.jimmunol.org/content/181/4/2772.long
Mast cells express IL-13R alpha 1: IL-13 promotes human lung mast cell proliferation and Fc epsilon RI expression.
Kaur D, Hollins F, Woodman L, Yang W, Monk P, May R, Bradding P, Brightling CE. Allergy. 2006 Sep;61(9):1047-53. onlinelibrary.wiley.com/doi/abs/10.1111/j.1398-9995.2006.01139.x
Neuroscience/Pharmacology
Inflammation and Brain Structure in Schizophrenia and Other Neuropsychiatric Disorders: A Mendelian Randomization Study.
Williams JA, Burgess S, Suckling J, Lalousis PA, Batool F, Griffiths SL, Palmer E, Karwath A, Barsky A, Gkoutos GV, Wood S, Barnes NM, David AS, Donohoe G, Neill JC, Deakin B, Khandaker GM, Upthegrove R; PIMS Collaboration. JAMA Psychiatry. 2022 May 1;79(5):498-507. doi: 10.1001/jamapsychiatry.2022.0407. https://jamanetwork.com/journals/jamapsychiatry/fullarticle/2790722
International Union of Basic and Clinical Pharmacology. CX. Classification of Receptors for 5-hydroxytryptamine; Pharmacology and Function.
Barnes NM et al. Pharmacol Rev. 2021 Jan;73(1):310-520. doi: 10.1124/pr.118.015552. https://pharmrev.aspetjournals.org/content/73/1/310.long
THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: G protein-coupled receptors.
Alexander SP, Christopoulos A, Davenport AP, Kelly E, Mathie A, Peters JA, Veale EL, Armstrong JF, Faccenda E, Harding SD, Pawson AJ, Southan C, Davies JA, Abbracchio MP, Alexander W, Al-Hosaini K, Bäck M, Barnes NM et al. Br J Pharmacol. 2021 Oct;178 Suppl 1:S27-S156. doi: 10.1111/bph.15538. https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15538
Design, synthesis and evaluation of tryptophan analogues as tool compounds to study IDO1 activity.
Cundy NJ, Hare RK, Tang T, Leach AG, Jowitt TA, Qureshi O, Gordon J, Barnes NM, Brady CA, Raven EL, Grainger RS, Butterworth S. RSC Chem Biol. 2021 Sep 13;2(6):1651-1660. doi: 10.1039/d0cb00209g. https://pubs.rsc.org/en/content/articlelanding/2021/CB/D0CB00209G
Novel mechanism of modulation at a ligand-gated ion channel; action of Cl-indole at the 5-HT3A receptor.
Powell AD, Grafton G, Roberts A, Larkin S, O’Neill N, Palandri J, Barnes NM. Br J Pharmacol 173, 3467-3479, 2016. bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.13638
Antioxidant, cytotoxic and anti-proliferative activities and total polyphenol contents of the extracts of Geissospermum reticulate bark.
Sajkowska-Kozielewicz J, Kozielewicz P, Barnes NM, Wawer I, Paradowska K. . Oxid Med Cell Longev doi: 10.1155/2016/2573580. www.hindawi.com/journals/omcl/2016/2573580/
The discovery of diazepinone-based 5-HT3receptor partial agonists.
Manning DD, Guo C, Zhang Z, Ryan KN, Naginskaya J, Choo SH, Masih L, Earley WG, Wierschke JD, Newman AS, Brady CA, Barnes, NM, Guzzo PR. Biorg Med Chem Lett 24, 2578-2581, 2014. www.sciencedirect.com/journal/bioorganic-and-medicinal-chemistry-letters/vol/24/issue/11
Cytokine function in medication-naïve first episode psychosis: a systematic review and meta-analysis.
Upthegrove R, Manzanares-Teson N, Barnes NM. Schizophrenia Res 155, 101-108, 2014 www.sciencedirect.com/science/article/pii/S0920996414001133
The immune system and schizophrenia: an update for clinicians.
Upthegrove, R, Barnes NM Adv Psych Treat 20, 83-91, 2014. pdfs.semanticscholar.org/3813/eb564ccf6a83644a740e4e2e2cb38fec0c3a.pdf
Detection of mRNA encoding receptors by in situ and northern hybridization.
Princivalle AP, Parker RM, Dover TJ, Barnes NM. Methods Mol Biol 897, 261-302, 2012.
Novel serotonin type 3 receptor partial agonists for the potential treatment of irritable bowel syndrome.
Manning DD, Cioffi CL, Usyatinsky A, Fitzpatrick K, Masih L, Guo C, Zhang Z, Choo SH, Sikkander MI, Ryan KN., Naginskaya J, Hassler C, Dobritsa S, Wierschke JD, Earley WG, Butler AS, Brady CA, Barnes NM, Cohen ML, Guzzo PR.
Bioorg Med Chem Lett. 21, 58-61, 2011. www.sciencedirect.com/science/article/pii/S0960894X10017051
The identification of N-glycosylation residues of the human 5-HT3B receptor subunit: importance for cell membrane expression.
Massoura AN, Dover TJ, Barnes NM. J. Neurochem. 116, 975-983, 2011. onlinelibrary.wiley.com/doi/abs/10.1111/j.1471-4159.2010.07129.x
Importance of the C-terminus of the human 5-HT3A receptor subunit.
Butler AS, Lindesay SA, Dover TJ, Kennedy MD, Patchell VB, Levine BA, Hope AG, Barnes NM. Neuropharmacology 56, 292-302, 2009. www.sciencedirect.com/science/article/pii/S0028390808003675?via%3Dihub
The 5-HT3 receptor – the relationship between structure and function.
Barnes NM, Hales TG, Lummis SC, Peters JA. Neuropharmacology 56, 273-284, 2009. www.sciencedirect.com/science/article/pii/S0028390808003407?via%3Dihub
Receptor Trafficking
Constitutive clathrin-mediated endocytosis of CTLA-4 persists during T cell activation.
Qureshi OS, Kaur S, Hou TZ, Jeffery LE, Poulter NS, Briggs Z, Kenefeck R, Willox AK, Royle SJ, Rappoport JZ, Sansom DM. J Biol Chem. 2012 Mar 16;287(12):9429-40. doi: 10.1074/jbc.M111.304329. Epub 2012 Jan 19. www.jbc.org/content/287/12/9429.long
Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors.
Royle SJ, Qureshi OS, Bobanović LK, Evans PR, Owen DJ, Murrell-Lagnado RD.J Cell Sci. 2005 Jul 15;118(Pt 14):3073-80. Epub 2005 Jun 28. jcs.biologists.org/content/118/14/3073.long