Home > Immunology Assays > T Cell Activation Assays

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Assays for measuring T cell activation, killing, proliferation, signalling and synapse formation

Celentyx can run high-throughput plate-based and  high-content assays of T cell activation (including on purified  T cell subsets) using a number of different readouts and performed on human primary cells either from healthy volunteers or patients with underlying disease:

• Proliferation measured by flow cytometry by dyes such as CFSE or equivalent and used to identify or characterise stimulatory or inhibitory molecules (A)
• Changes in expression of activation markers give an early indication of compound impact and can be easily combined with markers of T cell subsets (B)
• Clustering of T cells around target cells can be used to measure changes in adhesion or mobility (C)

Assays may be suitable for evaluating modulators of BiTEs, CD3, CD27, CD28, CD40, CD40L, CD70, CD80, CD86, CTLA-4, JAK1, JAK2, JAK3, ICOS, ICOSL, IL-2R, IL-9R, IL-21R, OX40, OX40L, PD-1, PDL-1, TIGIT, TIM-3, STAT1, STAT3, STAT5 and others.

Further Immunology Assays
B Cells
Haemolysis Testing
Human ​Microglia​
​Macrophages/Monocytes
​Neutrophils/Granulocytes
​Phagocytosis Assays
Regulatory T cells​
Spheroid Killing Assay​s
​Suppression Assays​​
T Cell Exhaustion Assays
Tumour infiltrating lymphocyte (TIL) and dissociated tumour cell assays​
Tumour Cell Killing Assays

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C.